Intracellular localization of DNA polymerase alpha.
نویسندگان
چکیده
منابع مشابه
Molecular architecture of the mouse DNA polymerase alpha-primase complex.
The DNA polymerase alpha-primase complex is the only enzyme that provides RNA-DNA primers for chromosomal DNA replication in eukaryotes. Mouse DNA polymerase alpha has been shown to consist of four subunits, p180, p68, p54, and p46. To characterize the domain structures and subunit requirements for the assembly of the complex, we constructed eukaryotic polycistronic cDNA expression plasmids exp...
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The mechanism by which millimolar concentrations of ATP stimulate the activity and increase the processivity of calf thymus DNA polymerase alpha has been investigated with poly(dA)/oligo(dT) as template/primer to eliminate possible effects due to primer synthesis. The effect of ATP on the rate of DNA synthesis with this template/primer was found to be dependent upon whether or not the ATP was n...
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DNA polymerase III is one of the five eubacterial DNA polymerases that is responsible for the replication of DNA duplex. Among the ten subunits of the DNA polymerase III core enzyme, the alpha subunit catalyzes the reaction for polymerizing both DNA strands. In this study, we extracted genomic sequences of the alpha subunit from 159 sequenced eubacterial genomes, and carried out sequence-based ...
متن کاملThe intranuclear localization of two DNA-dependent RNA polymerase activities.
DNA-dependent RNA polymerase of mammalian cells has been described by Weiss,' Huang, M\Iaheshwari, and Bonner,2 Goldberg,3 and Biswas and Abrams.4 The activity of RNA polymerase is enhanced if detergents are added to the incubation medium." I Goldberg3 found that ammonium salts at high ionic concentrations are particularly effective in stimulating the activity of the polymerase. Widnell and Tat...
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The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal of pol alpha and primase activities from crude extracts of HeLa cells or monkey cells by use of an anti-pol alpha immunoaffinity column resulted in the loss of replication activity. The addition of purified pol alpha-primase complex isolated f...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1981
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.78.5.3049